rat monoclonal antibody against cd3ε  (Bio-Rad)

Journal: Veterinary oncology (London, England)

Article Title: Intertumoral heterogeneity of the immune microenvironment in high grade canine mast cell tumors.

doi: 10.1186/s44356-025-00020-9

Figure Lengend Snippet: Fig. 3 Assessment of tumor infiltrating lymphocytes in canine MCTs. Correlative analyses of transcriptional T cell score with A. ICOSLG and B. PDCD1 transcripts (each dot represents an individual dog using log2 normalized expression of transcripts). Comparisons of C. transcriptional T cell score D. and density of CD3 + T cells determined immunohistochemically between low and high grade MCTs (mean and standard deviations displayed). Images of lymphoid aggregates consistent with tertiary lymphoid structures within the high grade MCT-12 using E. immunohistochemical staining against CD3 (DAB chromogen reaction and hematoxylin counterstain) and F. co-immunofluorescent labelling of CD3 (green), CD79b (red), KIT (white), nuclear DAPI (blue) labelling. (Scale bars = 200 μm. DAB- diaminobenzidine; DAPI- 4’,6’-diamidino-2-phenylinlindole. Correlative analyses performed using two-tailed Spearman correlation Spearman rho (rs) and p values displayed. Comparisons between two groups performed using two-tailed Mann–Whitney tests, ns = not significant.)

Article Snippet: A primary rat monoclonal antibody against CD3ε (CD3, Bio-Rad MCA1477T, clone CD312) was used at a concentration of 1/600 and incubated on the slides for 45 min at RT.

Techniques: Expressing, Immunohistochemical staining, Staining, Two Tailed Test, MANN-WHITNEY

Journal: Arthritis research & therapy

Article Title: Macrophage subpopulations in pediatric patients with lupus nephritis and other inflammatory diseases affecting the kidney.

doi: 10.1186/s13075-024-03281-1

Figure Lengend Snippet: Fig. 1 Immunofluorescence staining of inflammatory cells in pediatric kidneys. A CD68 + macrophages. B CD163 + cells. C Merge of CD68 + and CD163 + cells representing M2c-like macrophages. D CD68 + macrophages. E CD 206 + cells. F Merge CD68 + and CD206 + cells, representing M2a-like macrophages. G Renal T lymphocytes (CD3 + cells). H Renal B lymphocytes (CD20 + cells). I B and T Lymphocyte overlap (CD3 + and CD20 + cells. Scale bar represents 50 µm

Article Snippet: After blocking with normal goat serum and 1% blotto sections were incubated overnight at 4 °C using the following antibodies diluted in 1% BSA in 50 mM Tris(hydroxymethyl) aminomethan pH 7.6: iNOS, a rabbit polyclonal antibody against human iNOS (Abcam plc, Cambridge, UK); CD68, a mouse monoclonal IgG3 antibody against human CD68 (Dako Deutschland GmbH, Hamburg, Germany); CD163, a mouse monoclonal IgG1 antibody against human CD163 (Novocastra, Leica Biosystems Newcastle Ltd; Newcastle, UK); CD206, a mouse monoclonal IgG1 antibody against human CD206 (Abnova, Jhongli City, Taiwan); CD3, a monoclonal rat antibody against human CD3 (Bio-Rad AbD Serotec GmbH, Puchheim, Germany); CD20, a monoclonal mouse IgG2a antibody against human CD20 and MPO, a polyclonal rabbit antibody against myeloperoxidase (Abcam plc, Cambridge, UK).

Techniques: Immunofluorescence, Staining

Journal: Arthritis research & therapy

Article Title: Macrophage subpopulations in pediatric patients with lupus nephritis and other inflammatory diseases affecting the kidney.

doi: 10.1186/s13075-024-03281-1

Figure Lengend Snippet: Fig. 6 Clustering analysis of the abundance of inflammatory cells in different inflammatory kidney diseases in pediatric patients. Heatmap of the numbers of CD68 + CD163 + , CD68 + CD206 + , CD68 + CD163 − , CD68 + CD206 − , CD3 + , CD20 + , and MPO + cells in the analyzed patients (red: high number; blue: low number). The color-coded bar corresponding to the different rows (patients) indicates the diagnosis

Article Snippet: After blocking with normal goat serum and 1% blotto sections were incubated overnight at 4 °C using the following antibodies diluted in 1% BSA in 50 mM Tris(hydroxymethyl) aminomethan pH 7.6: iNOS, a rabbit polyclonal antibody against human iNOS (Abcam plc, Cambridge, UK); CD68, a mouse monoclonal IgG3 antibody against human CD68 (Dako Deutschland GmbH, Hamburg, Germany); CD163, a mouse monoclonal IgG1 antibody against human CD163 (Novocastra, Leica Biosystems Newcastle Ltd; Newcastle, UK); CD206, a mouse monoclonal IgG1 antibody against human CD206 (Abnova, Jhongli City, Taiwan); CD3, a monoclonal rat antibody against human CD3 (Bio-Rad AbD Serotec GmbH, Puchheim, Germany); CD20, a monoclonal mouse IgG2a antibody against human CD20 and MPO, a polyclonal rabbit antibody against myeloperoxidase (Abcam plc, Cambridge, UK).

Techniques: Biomarker Discovery

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Representative immunofluorescence microscopic images of TIGIT, CD3, and CD56 in BC tissues and adjacent tissues.

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques: Immunofluorescence

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Flow cytometry histograms of CD3 + and TIGIT + T cells in PBC patients ( A and B ) versus healthy controls ( C and D ).

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques: Flow Cytometry

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Comparison of TIGIT expression between two groups and the sensitivity of TIGIT for the diagnosis of PBC. ( A ) TIGIT level (%) on CD3 + T cells in PBC patients versus healthy controls. ( B ) The ROC curve drawn based on the percentage of TIGIT in peripheral blood T cells of PBC patients.

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques: Comparison, Expressing, Biomarker Discovery

Journal: International Journal of General Medicine

Article Title: Expression and Clinical Significance of TIGIT in Primary Breast Cancer

doi: 10.2147/IJGM.S407725

Figure Lengend Snippet: Correlation Between TIGIT Level on Peripheral Blood T Cells and Clinicopathological Features in PBC Patients

Article Snippet: Afterwards, the mixture was incubated with mouse monoclonal primary antibodies against CD3 (17A2#24,265; CST) and CD56 (123C3#3576; CST) overnight at 4°C.

Techniques: